Molecular Cloning of a Bovine Immunoglobulin Lambda Chain cDNA

A cDNA library of the bovine mammary gland constructed in pBR322 was screened by mRNA hybrid-selected translation and by differential hybridization. Several immunoglobulin (Ig) λ light-chain clones were identified and sequenced. Nucleotide sequence comparison of bovine and human Ig λ chains showed a high degree of homology for constant regions and for J regions. The amino acid (aa) sequence encoded by the constant region of the bovine Ig λ chain cDNA contains 107 aa with differences at 24 aa positions from the human Ig A chain. Three complementarity-determining regions (CDR1,2,3) characteristic of the variable region of bovine Ig λ chain cDNA can be distinguished. The bovine and human sequences display good homology in the framework region 3 (FR3) but only patches of homology throughout the FR2 region. The 5′ end of the bovine Ig λ chain cDNA fragment of clone 1-14E contains five stop codons: two in CDR1, one in FR1 and two in the hydrophobic prepeptide region. These data suggest that the Igλ mRNA of clone 1-14E is transcribed from the Vλ pseudogene

Exploiting Domain Knowledge in Making Delegation Decisions

@inproceedings{conf/admi/EmeleNSP11, added-at = {2011-12-19T00:00:00.000+0100}, author = {Emele, Chukwuemeka David and Norman, Timothy J. and Sensoy, Murat and Parsons, Simon}, biburl = {http://www.bibsonomy.org/bibtex/20a08b683088443f1fd36d6ef28bf6615/dblp}, booktitle = {ADMI}, crossref = {conf/admi/2011}, editor = {Cao, Longbing and Bazzan, Ana L. C. and Symeonidis, Andreas L. and Gorodetsky, Vladimir and Weiss, Gerhard and Yu, Philip S.}, ee = {http://dx.doi.org/10.1007/978-3-642-27609-5_9}, interhash = {1d7e7f8554e8bdb3d43c32e02aeabcec}, intrahash = {0a08b683088443f1fd36d6ef28bf6615}, isbn = {978-3-642-27608-8}, keywords = {dblp}, pages = {117-131}, publisher = {Springer}, series = {Lecture Notes in Computer Science}, timestamp = {2011-12-19T00:00:00.000+0100}, title = {Exploiting Domain Knowledge in Making Delegation Decisions.}, url = {http://dblp.uni-trier.de/db/conf/admi/admi2011.html#EmeleNSP11}, volume = 7103, year = 2011 }Postprin

Identification of Bacterial Clones Encoding Bovine Caseins by Direct Immunological Screening of the cDNA Library

A sensitive immunoassay was used to identify recombinant plasmids carrying cDNA fragments of bovine caseins in the cDNA library from bovine mammary gland mRNA. Colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto CNBr-activated cellulose filter paper. Antigens covalently bound to CNBr-activated paper or bound to nitrocellulose filters were detected by reaction with antiserum to caseins, followed by ^125I-labelled Staphylococcus aureus protein A and autoradiography. Six clones were found positive among 5400 of the cDNA library: 3-A1, 3-B2, 3-B5, 3-H7, 2-A5 and 2-C9. The molecular weights of chimeric pre-β-lactamase: casein proteins synthesized in Escherichia coli were estimated by immunoblotting. Colony hybridization and nucleotide sequence analysis showed that clone 3-B5 contained a cDNA fragment of bovine χ-casein, clone 3-H7 contained a cDNA fragment of β-casein, while clones 2-A5 and 2-C9 carried cDNA fragments of α_si-casein

Identification of Bacterial Clones Encoding Bovine Caseins by Direct Immunological Screening of the cDNA Library

A sensitive immunoassay was used to identify recombinant plasmids carrying cDNA fragments of bovine caseins in the cDNA library from bovine mammary gland mRNA. Colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto CNBr-activated cellulose filter paper. Antigens covalently bound to CNBr-activated paper or bound to nitrocellulose filters were detected by reaction with antiserum to caseins, followed by ^125I-labelled Staphylococcus aureus protein A and autoradiography. Six clones were found positive among 5400 of the cDNA library: 3-A1, 3-B2, 3-B5, 3-H7, 2-A5 and 2-C9. The molecular weights of chimeric pre-β-lactamase: casein proteins synthesized in Escherichia coli were estimated by immunoblotting. Colony hybridization and nucleotide sequence analysis showed that clone 3-B5 contained a cDNA fragment of bovine χ-casein, clone 3-H7 contained a cDNA fragment of β-casein, while clones 2-A5 and 2-C9 carried cDNA fragments of α_si-casein

Dual-Resonator Speed Meter for a Free Test Mass

A description and analysis are given of a ``speed meter'' for monitoring a classical force that acts on a test mass. This speed meter is based on two microwave resonators (``dual resonators''), one of which couples evanescently to the position of the test mass. The sloshing of the resulting signal between the resonators, and a wise choice of where to place the resonators' output waveguide, produce a signal in the waveguide that (for sufficiently low frequencies) is proportional to the test-mass velocity (speed) rather than its position. This permits the speed meter to achieve force-measurement sensitivities better than the standard quantum limit (SQL), both when operating in a narrow-band mode and a wide-band mode. A scrutiny of experimental issues shows that it is feasible, with current technology, to construct a demonstration speed meter that beats the wide-band SQL by a factor 2. A concept is sketched for an adaptation of this speed meter to optical frequencies; this adaptation forms the basis for a possible LIGO-III interferometer that could beat the gravitational-wave standard quantum limit h_SQL, but perhaps only by a factor 1/xi = h_SQL/h ~ 3 (constrained by losses in the optics) and at the price of a very high circulating optical power --- larger by 1/xi^2 than that required to reach the SQL.Comment: RevTex: 13 pages with 4 embedded figures (two .eps format and two drawn in TeX); Submitted to Physical Review

MULTI-AGENT MODELING AND SIMULATION OF DISTRIBUTED DENIAL-OF-SERVICE ATTACKS ON COMPUTER NETWORKS

Переход к реализации военно-морских операций, базирующихся на современной парадигме использования информационно-телекоммуникационного пространства, имеет несомненное преимущество, связанное с применением распределенной компьютерной обработки информации для получения превосходства над противником. Однако, противник будет пытаться атаковать информационные инфраструктуры, используемые силами флотов для выполнения военно-морских операций. Одно из действенных средств, приводящих к нарушению работы этих инфраструктур, —это распределенные компьютерные атаки типа “Отказ в обслуживании ” (DDoS-атаки). Основная цель таких атак состоит в нарушении или снижении возможностей доступа авторизованных пользователей к распределенным вычислительным ресурсам, а также их компрометации. Повышение живучести информационных систем и инфраструктур в условиях реализации противником таких атак требует разработки адекватного теоретического и практического фундамента. Одной из важных составляющих такого фундамента является наличие средств моделирования DDoS-атак. Формальный подход к моделированию полного спектра атак данного класса, ключевыми элементами которого является онтология DDoS-атак, механизмы командной работы программных агентов, реализующих DDoS-атаки, а также программный инструментарий для разработки многоагентных систем MASDK, используемый дл

Agents and Data Mining Interaction8th International Workshop, ADMI 2012, Valencia, Spain, June 4-5, 2012, Revised Selected Papers /

XII, 217 p. 81 illus.online resource

Agents and Data Mining Interaction7th International Workshop on Agents and Data Mining Interation, ADMI 2011, Taipei, Taiwan, May 2-6, 2011, Revised Selected Papers /

XIII, 361 p.online resource